Deep phenotypic and cytotoxicity characterization of NK cells cultured with chemically defined additives

Cell & Gene Therapy Insights 2025; 11(8), 1045

DOI: 10.18609/cgti.2025.120

Published: 3 October
FastFacts
Rebecca Nickle


 

Immunotherapy is transforming cancer treatment by harnessing the body’s immune system, with natural killer (NK) cells emerging as promising alternatives to T cells due to their innate cytotoxicity. However, delivering effective NK cell therapies highly depends on the ancillary materials used during ex vivo bioprocessing and robust cell characterization. This poster outlines a workflow from NK cell isolation to serum-free culture and cytotoxic analysis, offering a comprehensive view of NK cell function through targeted killing, surface marker profiling, and secreted molecule characterization.


This 10-minute video presentation, accompanied by a poster summary, outlines a workflow from NK cell isolation to serum-free culture and cytotoxic analysis, offering a comprehensive view of NK cell function.

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