Achieving high-titer rAAV production with significantly reduced encapsidated host cell DNA

Live30 webinars are thirty-minute presentations designed to update you on the latest innovations, applications, and data in a fast yet interactive format.

Attend this webinar to learn about high-titer rAAV production by transient transfection, with high packaging efficiency and significantly reduced levels of encapsidated hcDNA.

The speaker will present and review case study data detailing a transient cell line for high-titer rAAV productivity and high packaging efficiency across multiple serotypes, with reproducible and scalable bioreactor process performance from the 15 mL to 10 L scale. A 100-fold reduction in encapsidated hcDNA (≤ 12.4 ng/10¹⁴ vg) was achieved.

Attend this webinar to learn more about:

• The continued importance of transient transfection cell lines during R&D and in clinical and commercial production
• How cell line engineering can address encapsidated host cell DNA , an impurity which is resistant to nucleases and chromatographic clearance
• Data on AAV productivity and scalability across serotypes

Ines Goncalves

Head of Project Management at Cytiva

Ines Goncalves has held several scientific and management positions in the gene therapy and biotechnology sector. During her career, she has worked on advancing the development of stable cell lines for viral vector production and collaborated with global clients to pioneer innovative solutions for gene therapy manufacturing. Ines’s academic journey began with a Bachelor´s and Master´s degree in Molecular Biology and Genetics from the University of Lisbon, Portugal. She then pursued a PhD in Genetics at the University Hospital of Cologne, Germany, where her research focused on Neurobiology, specifically on spinal muscular atrophy. Recently, Ines completed an MBA degree from WHU – Otto Beisheim School of Management in Germany.

Watch now

SPEAKERS

Ines Goncalves

Head of Project Management at Cytiva

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