Achieving scalable, high-yield plasmid DNA purification processes

Thursday 08:00 PDT / 11:00 EDT / 16:00 BST / 17:00 CEST

Sponsor

Achieving scalable, high-yield plasmid DNA purification processes

Join this webinar to explore how to overcome key bottlenecks in plasmid (p)DNA downstream processing.

Using real-world process data, the session will explore how to optimize each step—from lysis to sterile filtration—for maximum yield, purity, and regulatory compliance.

The webinar will feature case studies demonstrating a fully integrated, end-to-end pDNA purification process achieving

>98% integrity and >64% process recovery of supercoiled pDNA
>95% sc pDNA in lysate using automated in-line lysis
>90% recovery rates for both AEX and HIC steps
<4% loss in final UF/DF and sterile filtration

Plus, we demonstrate real-time process monitoring using at-line analytics to fine-tune performance and ensure quality

Join the webinar to learn how to:

Overcome scale-up challenges in pDNA production, including control over lysis and chromatographic separation at large scale
Maximize recovery and consistency at gram-scale while meeting regulatory standards
Implement continuous plasmid purification to preserve pDNA quality
Leverage at-line analytics to monitor and optimize purification in real time

Klemen Božič

Scientist, Process Development mRNA/pDNA at Sartorius BIA Separations

Klemen holds a MPharm degree from the University of Ljubljana, Slovenia. In his first role in Slovenian OMCL, he gained experience on analytics of commercially available drugs. In 2020 he joined mRNA/pDNA Process Development department at Sartorius BIA Separations. He has extensive experience in pDNA analytics and focuses on continuous improvement of pDNA downstream process.

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