Key strategies for pDNA optimization and scale-up to 30L

Tuesday 08:00 PDT / 11:00 EDT / 16:00 BST / 17:00 CEST

Sponsor

Key strategies for pDNA optimization and scale-up to 30L

This webinar will provide a framework for designing scalable pDNA production workflows using design of experiments (DoE) methodology. The session will feature analysis of real-world data from a 4-factor, 2-level DoE screening of vessel type, media, feed, and carbon source. Additional data highlights include a comparison of 1 L and 30 L fermentations conducted in the same bioreactor, as well as a comparative analysis of media types [Luria Bertani (LB) vs Terrific Broth (TB)] and carbon sources (glycerol vs glucose) on pDNA yield and cell growth performance.

We’ll also address common challenges in scaling pDNA production while maintaining product quality. Topics will include defining benchmarks for yield optimization at different production scales, preparing for technology transfer, and how Fast Trak™ process development services from Cytiva can accelerate your pipeline.

Attend this webinar to gain:

A clear understanding of how to optimize key culture parameters to maximize pDNA yield
Strategies and insights for scaling pDNA production from benchtop to pilot scale
Practical guidance and success stories you can learn from, demonstrating how others have scaled their production efficiently

Denis Kole

Director of Fast Trak™ Process Development Services

Dr. Denis Kole is Director of Fast Trak™ Process Development Services, at Cytiva in the US. Denis has over 20 years of experience in the biopharmaceutical field. In his current role he leads the technical teams responsible for the execution of PD and scale-up operations for biologics and advanced therapy medicinal products (ATMP). He has established a significant track record in leading and completing projects related to biologics drug discovery, viral vector processes, vaccine development, and bioprocess intensification. In the past 5 years, Dr. Kole has been working with over 30 biotechnology, biopharma, and academic laboratories to consult, develop, optimize, scale-up, and tech-transfer bioprocesses from bench-scale to commercially viable processes.

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