Adenylator DNA/RNA 5΄-Adenylation Kit: clean, efficient enzymatic adenylation of DNA and RNA

Adenylation of 5′-monophosphorylated oligonucleotides is a critical enzymatic step in the preparation of sequencing libraries and adapter-linked constructs, particularly in small RNA sequencing (small RNA-seq) and native elongating transcript sequencing (NET-seq) workflows. This modification enables subsequent ligation steps by generating a pre-adenylated intermediate, which is required for efficient and directional ligation. Traditionally, this is achieved using thermostable RNA ligases such as Mth RNA Ligase, which requires an elevated reaction temperature of 65 °C and can produce unwanted ligation side products, such as concatemers or circularized oligos.  The Adenylator™ DNA/RNA 5′-Adenylation Kit offers a novel alternative by utilizing a non-ligating, high-efficiency enzyme that specifically catalyzes the 5′-adenylation of both DNA and RNA oligonucleotides under mild conditions (37 °C) and without formation of circularized oligo and concatemerization byproducts. This poster evaluates the enzymatic activity and substrate specificity of the Adenylator Enzyme and provides a direct performance comparison with Mth RNA Ligase under standardized assay conditions. NEW INTERACTIVE FORMAT Listen to our expert speaker. Watch the video. Read the summary. Focus in on the data. Download the PDF. It has never been easier to quickly access the information you want. All in one place—at the click of a button.

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