Process optimization of mRNA purification for vaccines and therapeutic applications
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Live30 webinars are thirty-minute presentations designed to update you on the latest innovations, applications, and data in a fast yet interactive format.
The success of mRNA vaccines in combating a global pandemic highlights the power of this technology not only to revolutionize prophylactic treatments for infectious diseases, but also to treat other diseases such as cancer. Critical to bringing these medicines to patients is an efficient, effective, and scalable downstream production process.
One of the molecular hallmarks of mRNA is its 3-prime poly-A tail, consisting of many consecutive adenylate bases. Leveraging fundamental A:T base complementarity, an affinity resin was developed to simplify affinity capture of in vitro transcribed mRNA.
This webinar will address the development, characterization, and optimization of an mRNA capture step using POROS Oligo(dT)25 resin. mRNA research techniques have developed over several decades, and associated traditional purification methods have proven challenging to scale. A newer affinity solution leveraging the rigid POROS backbone with its linear pressure-flow relationship enables a consistent purification platform, from process development through to clinical manufacturing.
- Considerations for selection of affinity capture step in the downstream purification of mRNA
- Performance data of the POROS Oligo(dt)25 affinity resin under various conditions and feeds
- Practical approaches via Design of Experiments (DoE) to optimize binding capacity and yield an effective capture step for mRNA vaccines and therapeutics
- Effective strategies to control cost of goods including resin cleaning and re-use conditions, and performance data
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