mRNA characterization - from 5’ cap to poly (A): what IPRP-LC–MS can tell you
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mRNA is a biotherapeutic modality primarily used in vaccines, and undergoes a complex manufacturing process involving in vitro transcription. This process yields mRNA molecules exceeding 1,000 nucleotides in length, with a 5' cap and a poly (A) tail. To ensure the desired product quality of mRNA therapeutics, critical quality attributes must be maintained within appropriate limits. Therefore, the implementation of robust analytical methods is crucial for making quality decisions.
Attend this webinar for insights into an mRNA characterization workflow that includes sample preparation, ion-pairing reversed-phased liquid chromatography-mass spectrometry (IPRP-LC-MS) methodology, and bioinformatics processing.
The workflow evaluates 5' cap efficiency, maps oligonucleotide sequence fragments, resolves isomers, and analyzes the intact mass of the poly (A) tail.
Attend this webinar to:
- Explore the LC-MS characterization method for 5' cap, coding sequence mapping, and poly (A) tail analysis
- Gain insights into utilizing ion-pairing reversed-phase LC-MS to characterize mRNA drug substances
- Discover the benefits of utilizing BioTi core-shell column technology to improve sensitivity and resolution for oligonucleotide identification
- Review the bioinformatics processing workflow
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